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Table 2 Primer sequences used in real-time PCR (qPCR)

From: Leucine alters hepatic glucose/lipid homeostasis via the myostatin-AMP-activated protein kinase pathway - potential implications for nonalcoholic fatty liver disease

Gene name Primers Primer sequences
SLC2A2 Sense 5'- CATTCCAATTAGAAAGAGAGAACGTC-3'
Antisense 5'-AGCAAACCTGTTTATGCAACC-3'
G6Pase Sense 5'-TACGTCCTCTTCCCCATCTG-3'
Antisense 5'-CCTGGTCCAGTCTCACAGGT-3'
PCK1 Sense 5'-GGTTCCCAGGGTGCATGAAA-3'
Antisense 5'-CACGTAGGGTGAATCCGTCAG-3'-3'
PC Sense 5'-TTGCCCACTTCAAGGACTTC-3'
Antisense 5'-CTTTGATGTGCAGCGTCTTG-3'
FOXO1 Sense 5’-GCTGCATCCATGGACAACAACA-3'
Antisense 5’-CGAGGGCGAAATGTACTCCAGTT-3'
PPARGC1A Sense 5'-TGTGCAACTCTCTGGAACTG-3'
Antisense 5'-TGAGGACTTGCTGAGTGGTG-3'
MSTN Sense 5'-CGTCTGGAAACAGCTCCTAACA-3'
Antisense 5'-GAAAATCAGACTCTGTAGGCATGGT-3'
GPAT1 Sense 5’-AACCCCAGTATCCCGTCTTT-3’
Antisense 5’-CAGTCACATTGGTGGCAAAC-3’
FOXA2 Sense 5-TGTTCATGCCGTTCATCCC-3
Antisense 5-GGAGCGGTGAAGATGGAAG-3
GK Sense 5'-GATGCACTCAGAGATGTAGTCG-3'
Antisense 5'-TGAAGGTGGGAGAAGGTGAG-3'
ACC1 Sense 5’-ATCCCG TACCTTCTTCTACTG-3’
Antisense 5’-CCCAAACATAAGCCTTCACTG-3’
PPAR γ Sense 5'-CCACTATGGAGTTCATGCTTGTGAAGG-3'
Antisense 5'-TGCAGCGGGGTGATGTGTTTGAACTTG-3'
PPIA Sense 5'-TCTTGAGGGAAGCATATTGG-3'
  Antisense 5'-CAGGGAGACTGACTGTAGCAC-3'