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Table 4 Primers used for PCR methods

From: Vitamin and antioxidant rich diet increases MLH1 promoter DNA methylation in DMT2 subjects

Primer name

Sequence 5′-3′

Application

Size

Number of CpGs

Ref

MLH1-C-FW

GTATTTTTGTTTTTATTGGTTGGATA

COBRA

294 bp

1

[24]

MLH1-C-RE

AATACCTTCAACCAATCACCTCAATA

COBRA

   

MLH1-1-FW

GGAGAGGAGGAGTTTGAGAAG

PSQ

269 bp

7

 

MLH1-1-RE

Biotin-AATACCAATCAAATTTCTCAACTCTAT

PSQ

   

MLH1-1-Seq

TTGTTTTTATTGGTTGGATAT

PSQ

   

MGMT-FW

Biotin-GGATATGTTGGGATAGTT

PSQ

266 bp

5

[42]

MGMT-RE

AAACTAAACAACACCTAAA

PSQ

   

MGMT-Seq

CCCAAACACTCACCAAA

PSQ

   

MLH1-Q-FW

TTCTCAGGTTATCGGAGCCAGCAC

qRT-PCR

288 bp

 

[43]

MLH1-Q-RE

CTTCGTCCCAATTCACCTCAGTGG

qRT-PCR

   

DNMT1-Q-FW

ACCGCTTCTACTTCCTCGAGGCCTA

qRT-PCR

335 bp

 

[44]

DNMT1-Q-RE

GTTGCAGTCCTCTGTGAACACTGTGG

qRT-PCR

   

GAPDH-Q-FW

CGACCACTTTGTCAAGCTCA

qRT-PCR

205 bp

 

[45]

GAPDH-Q-RE

AGGGGTCTACATGGCAACTG

qRT-PCR

   
  1. COBRA, combined bisulfite restriction analysis: PSQ, pyrosequencing: qRT-PCR, quantitative real-time reverse-transcriptase polymerase chain reaction; Ref, reference.