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Fig. 4 | Clinical Epigenetics

Fig. 4

From: ABT-737 and/or folate reverse the PDGF-induced alterations in the mitochondrial apoptotic pathway in low-grade glioma patients

Fig. 4

Overexpression of Bcl-w and Bcl-xl in Ntv-a/PDGF cells participates to the low level of Bax activation in Ntv-a/PDGF cells. a Effect of the folate or PDGFri treatment on the expression levels of anti-apoptotic proteins. Actin is used as loading control. b Relative BimmRNA and PUMAmRNA expressions were estimated by qPCR. Cells were treated with NFκBi: BAY11 (Calbiochem, France) at 100 μM for 42 h. c Effect of a range of the NFκBi (BAY11, 50, and 100 μM) treatment on the subcellular localization of NFκB and on the Bcl-w and Bcl-xl mRNA expression. Nuclear extraction was performed using the Nuclear Extract kit (Active Motif, France). GAPDHs are used as control in the RT-PCR analysis. d Correlation between the transcriptional activity of NFκB, the phosphorylation level of IκB, and the Akt activity in Ntv-a/PDGF cells treated or not with folate, PDGFri or Akti

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