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Table 4 Global DNA methylation in tissues from ApoE mice fed a control (C) or high fat (HF) diet depleted of folic acid (−F) or folic acid and vitamins B6 and B12 (−F−B) for 16 weeks

From: Differential effects of nutritional folic acid deficiency and moderate hyperhomocysteinemia on aortic plaque formation and genome-wide DNA methylation in vascular tissue from ApoE-/- mice

Global DNA methylation Control fat High fat
C C−F C−F−B HF HF−F HF−F−B
Aorta 5-methyldeoxycytidine (ng/μg DNA) 125.5 ± 8.2 (6) 135.7 ± 7.7 (6) 134.4 ± 22. (6) 116.6 ± 11.5 (6) 123.7 ± 7.8 (6) 133.2 ± 15.7 (6)
DNA methylation (%) 3.72 ± 0.06 (6) 3.61 ± 0.05 (6) 3.62 ± 0.07 (6) 3.60 ± 0.05 (6) 3.57 ± 0.04 (6) 3.58 ± 0.07 (6)
Heart 5-methyldeoxycytidine (ng/μg DNA) 158.9 ± 10.9 (6) 135.3 ± 4.8 (6) 130.8 ± 3.7 (6) 134.7 ± 6.1 (6) 157.0 ± 13.4 (6) 148.8 ± 9.8 (6)
DNA methylation (%) 3.8 ± 0.08 (6) 3.68 ± 0.11 (6) 3.75 ± 0.04 (6) 3.79 ± 0.05 (6) 3.85 ± 0.12 (6) 3.81 ± 0.05 (6)
Aorta tunica adventitia 5-methyldeoxycytidine (ng/μg DNA) 137.9 ± 12.2 (9) 141.0 ± 8.6 (10) 184.9 ± 22.3 (10) 171.5 ± 13.0 (8) 149.7 ± 20.1 (9) 157.8 ± 16.2 (7)
DNA methylation (%) 4.05 ± 0.11 (10) 3.94 ± 0.15 (10) 4.25 ± 0.10 (10) 4.31 ± 0.12 (8) 3.99 ± 0.10 (9) 3.98 ± 0.13 (7)
Liver 5-methyldeoxycytidine (ng/μg DNA) 68.1 ± 5.5 (10) 66.6 ± 4.7 (10) 71.4 ± 5.3 (10) 71.5 ± 5.9 (10) 68.4 ± 6.5 (10) 67.6 ± 6.6 (10)
DNA methylation (%) 3.19 ± 0.04 (10) 3.20 ± 0.04 (10) 3.21 ± 0.05 (10) 3.21 ± 0.05 (10) 3.20 ± 0.06 (10) 3.21 ± 0.05 (10)
  1. Values are means ± SEM with the number of mice sampled per group in parentheses